doi: 10.17586/2226-1494-2020-20-2-185-192


DIFFUSE REFLECTION ELECTRON SPECTROSCOPY IN STUDY OF BLOOD SERUM WITH MULTIPLE MYELOMA

L. V. Plotnikova, A. P. Nechiporenko, A. D. Garifullin, A. Y. Kuvshinov, S. V. Voloshin


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Plotnikova L.V., Nechiporenko A.P., Garifullin A.D., Kuvshinov A.Yu., Voloshin S.V. Diffuse reflection electron spectroscopy in study of blood serum with multiple myeloma. Scientific and Technical Journal of Information Technologies, Mechanics and Optics, 2020, vol. 20, no. 2, pp. 185–192 (in Russian).
doi: 10.17586/2226-1494-2020-20-2-185-192


Abstract
Subject of Research. The paper presents testing and evaluation of the spectral method potential capabilities in relation to the study of human blood serum in normal and in patients with multiple myeloma. The study of samples of native and lyophilized serum is carried out in comparison with the spectral characteristics of aqueous extracts of animal muscle sarcoplasm. Method. A non-destructive diffuse reflection electron spectroscopy method with a vertical beam path in the wavelength range of 200–700 nm in the absorption format was used. Main Results. In contrast to the native donor serum, a band of mucopolysaccharides (410–415 nm) of different intensity has been observed in the spectra of samples of animal tissue sarcoplasm extracts. This band appeared in the spectra of donor serum after lyophilization. That is why the presence of the band of mucopolysaccharides in the patients’ serum spectra is associated with reduced moisture binding capacity of the protein-polysaccharide complex, which, in turn, is due to the features of protein secondary structure in different forms of multiple myeloma. Practical Relevance. The peculiarities in the similarities and differences between blood serum spectra of donors and patients give reason to believe that the method of diffuse reflection electron spectroscopy, that does not require a lot of material and preliminary sample preparation, can be promising as a rapid tool for additional initial diagnosis and the treatment process monitoring of multiple myeloma and other lymphoproliferative diseases.

Keywords: multiple myeloma, diffuse reflection electron spectroscopy

References
  1. Kochubey V.I., Bashkatov A.N. Spectroscopy of Scattering Media. Tutorial. Saratov, Novy Veter Publ., 2014, 87 p. (in Russian)
  2. Orehova S., Nechiporenko U., Vasileva I., Nechiporenko A. Electronic spectrum of pork and beef muscle tissue surface samples, subjected to electron-radiation processing // Proc. 6th Baltic Conference on Food Science and Technology: Innovations for Food Science and Production (FOODBALT-2011), Latvia, Jelgava, 2011, Р. 199–203.
  3. Plotnikova L.V., Nechiporenko A.P., Orekhova S.M., Plotnikov P.P., Ishevskii A.L. A study of muscular tissue of animal origin by reflection-spectroscopy methods. Optics and Spectroscopy, 2017, vol. 122, no. 6, pp. 1015–1018. doi: 10.1134/S0030400X17060157
  4. Nechiporenko A.P., Nechiporenko U.Yu., Plotnikova L.V., Podshivalov A.V., Myakin S.V, Uspenskaya M.V. Effect of lyophilization and various treatments on spectral characteristics of beef muscle tissue and its main components. Journal of Analytical Chromatography and Spectroscopy, 2018, vol. 1, no. 2. doi: 10.24294/jacs.v1i2.494
  5. Nechiporenko A.P., Nechiporenko U.Yu., Konovalenko A.V., Myakin S.V. Characterization of liquid and solid biological tissues by diffused reflectance spectroscopy. Applied Solid State Chemistry, 2018, no. 2, pp. 22–29. doi: 10.18572/2619-0141-2018-2-3-22-29
  6. Saidov G.V., Sverdlova O.V. Molecular Spectroscopy Methods. St.Petersburg, Professional Publ., 2008, 338 p. (in Russian)
  7. Antipova L.V., Glotova I.A., Rogov I.A. Methods of Meat and Meat Products Research. Moscow, Kolos Publ., 2001, 376 p. (in Russian)
  8. Nechiporenko U.Yu., Plotnikova L.V., Melnikova M.I. Lipids, Their Blends, Oil Extracts and Extracted Meal of Vegetable Raw Materials. Optical Characteristics. LAP Lambert Academic Publishing RU, 2019, 149 p. (in Russian)
  9. Demchenko A.P. Ultraviolet Spectrophotometry and Protein Structure. Kiev, Naukova Dumka Publ., 1981, 208 p. (in Russian)
  10. Radiation Chemistry of Major Food Components: Its Relevance to the Assessment of the Wholesomeness of Irradiated Foods/ ed. by P.S. Elias, A.J. Cohen. Elsevier, 1977, 220 p.
  11. Sokolov A.A. Physico-Chemical and Biochemical Fundamentals of Meat Products Technology. Moscow, Food Industry Publ., 1965, 490 p. (in Russian)
  12. Kyle R.A., Gertz M.A., Witzing T.E., Lust J.A., Lacy M.Q., Dispenzieri A., Fonseca R., Rajkumar S.V., Offord J.R., Larson D.R., Plevak M.E., Therneau T.M., Greipp P.R. Review of 1027 patients with newly diagnosed multiple myeloma. Mayo Clinic Proceedings, 2003, vol. 78, no. 1, pp. 21–33. doi: 10.4065/78.1.21
  13. Multiple Myeloma. Diagnosis and Treatment/ ed. by M.A. Gertz, S.V. Rajkumar. New York, Springer, 2014, 311 p. doi: 10.1007/978-1-4614-8520-9
  14. Voitsekhovsky V.V., Landyshev Yu.S., Grigorenko A.A., Tseluiko S.S., Goborov N.D. Multiple Myeloma. Modern Principles of Diagnosis and Treatment. Blagoveshchensk, Poly-M Publ., 2012, 140 p. (in Russian)
  15. Abdulkadyrov K.M., Bessmeltsev S.S., Voloshin S.V., Chechetkin A.V., Martynkevich I.S. et.al. Traditional and New Approaches to Diagnosis, Prognosis, and Treatment of Multiple Myeloma. St.Petersburg, NIGHT Publ., 2015, 69 p. (in Russian)
  16. Кyle R.A., Rajkumar S.V. Criteria for diagnosis, staging, risk stratification and response assessment of multiple myeloma. Leukemia, 2009, vol. 23, no. 1, pp. 3–9. doi: 10.1038/leu.2008.291
  17. Sverdlova O.V. Electronic Spectra in Organic Chemistry. Leningrad, Chemistry Publ., 1985, 248 p. (in Russian)
  18. Nechiporenko A.P., Uspenskaya M.V., Gredyuhina I.V., Plotnikova L.V., Balbekin N.S. The impact of the acrylic acid neutralization degree and binding agent on the properties of polyacrylate systems. Part II. Scientific Journal NRU ITMO. Processes and Food Production Equipment, 2017, no. 1(31), pp. 26–35. (in Russian). doi: 10.17586/2310-1164-2017-10-1-26-35
  19. Gruzin P.G., Sorokovnikov V.A., Semibratova V.A. Ultraviolet absorption spectroscopy of cerebrospinal fluid. Part I. Characteristics of absorbing spectrum and normative data of spectroscopy at the study of cerebrospinal fluid albumen of patients without deceases of spine, spinal cord and its roots. Bulletin of the East Siberian Scientific Center SB RAMS, 2010, № 6-1(76), pp. 36–41. (in Russian)
  20. Katzmann L.A., Abraham R.S., Dispenzieri A., Lust J.A., Kyle R.A. Diagnostic performance of quantitative κ and λ free light chain assays in clinical practice. Clinical Chemistry, 2005, vol. 51, no. 5, pp. 878–881. doi: 10.1373/clinchem.2004.046870


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