APPLICATION OF CHEMOMETRICS FOR ANALYSIS OF BIOAEROSOLS BY FLOW-OPTICAL METHOD
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For citation: Khudyakov E.S., Kochelaev E.A., Volchek A.O., Kirsanov D.O., Jahatspanian I.E. Application of chemometrics for analysis of bioaerosols by flow-optical method. Scientific and Technical Journal of Information Technologies, Mechanics and Optics, 2016, vol. 16, no. 1, pp. 30–38.
Subject of Research. The informativity of detection channels for bioaerosol analyzer is investigated. Analyzer operation is based on flow-optical method. Method. Measurements of fluorescence and the light scattering of separate bioaerosol particles were performed in five and two spectral ranges, correspondingly. The signals of soil dust particles were registered and used as an imitation of background atmospheric particles. For fluorescenceinduction of bioaerosol particles we used light sources: a laser one with a wavelength equal to 266 nm and 365 nm LED source.Main Results. Using chemometric data processing the classification of informative parameters has been performed and three most significant parameters have been chosen which account for 72% of total data variance. Testing has been done using SIMCA and k-NN methods. It has been proved that the use of the original and the reduced sets of three parameters produces comparable accuracy for classification of bioaerosols. Practical Relevance. The possibility of rapid detection and identification of bioaerosol particles of 1-10 microns respirable fraction (hindering in the human respiratory system) by flow-optical method on a background of non-biological particles is demonstrated. The most informative optical spectral ranges for development of compact and inexpensive analyzer are chosen.
Acknowledgements. This work was partially financially supported by the Government of the Russian Federation (Grant 074-U01).
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