DOI: doi:10.17586/2226-1494-2015-15-2-275-284


V. S. Sibirtsev, L. V. Krasnikova, . , S. A. Stroev, I. A. Naumov, R. O. Olekhnovich, V. F. Tereschenko, E. M. Shabanova, M. Al-Khatib

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For citation: Sibirtsev V.S., Krasnikova L.V., Schleikin A.G., Stroev S.A., Naumov I.A., Olekhnovich R.O., Tereschenko V.F., Shabanova E.M., Mussa Al-Khatib. New biotesting method with the application of modern impedance technologies. Scientific and Technical Journal of Information Technologies, Mechanics and Optics, 2015, vol.15, no. 2, pp. 275–284.


The paper deals with new concepts of biotesting method updating. Modern conductometric technologies and the analysis of microbial «growth curves» are used. The registration occurs in a real time mode for the set of parallel samples. Results are shown for comparison of the proposed impedance biotesting technique with standard cultural determination method for total amount of microorganismes in the tested samples. Results are presented for practical application of the proposed impedance biotesting technique to the analysis as inhibitory action of clorhexidine disinfectant on the vital activity of Escherichia coli, as milk ripening process at the presence of various microorganisms species and protein preparations. The impedance biotesting method, proposed in the present work, provides high level of its own data convergence with the data, being received as a result of application of standard cultural biotesting techniques. Thus, the proposed method has such advantages, as: an opportunity of the detailed information reception about dynamics change of magnitude of population and intensity of test microorganisms metabolism, significant reduction of the culture media amount used, as well as researcher's temporary and labor efforts while the analyses realization, and the growth of analysis objectivity.

Keywords: biotesting, conductivity measurement, decontaminate activity, impedance analysis of microorganisms, microbiological growth curves, transglutaminaza, milk ripening

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