DOI: doi:10.17586/2226-1494-2015-15-2-275-284


NEW BIOTESTING METHOD WITH THE APPLICATION OF MODERN IMPEDANCE TECHNOLOGIES

V. S. Sibirtsev, L. V. Krasnikova, . , S. A. Stroev, I. A. Naumov, R. O. Olekhnovich, V. F. Tereschenko, E. M. Shabanova, M. Al-Khatib


Read the full article 
Article in Russian

For citation: Sibirtsev V.S., Krasnikova L.V., Schleikin A.G., Stroev S.A., Naumov I.A., Olekhnovich R.O., Tereschenko V.F., Shabanova E.M., Mussa Al-Khatib. New biotesting method with the application of modern impedance technologies. Scientific and Technical Journal of Information Technologies, Mechanics and Optics, 2015, vol.15, no. 2, pp. 275–284.

Abstract

The paper deals with new concepts of biotesting method updating. Modern conductometric technologies and the analysis of microbial «growth curves» are used. The registration occurs in a real time mode for the set of parallel samples. Results are shown for comparison of the proposed impedance biotesting technique with standard cultural determination method for total amount of microorganismes in the tested samples. Results are presented for practical application of the proposed impedance biotesting technique to the analysis as inhibitory action of clorhexidine disinfectant on the vital activity of Escherichia coli, as milk ripening process at the presence of various microorganisms species and protein preparations. The impedance biotesting method, proposed in the present work, provides high level of its own data convergence with the data, being received as a result of application of standard cultural biotesting techniques. Thus, the proposed method has such advantages, as: an opportunity of the detailed information reception about dynamics change of magnitude of population and intensity of test microorganisms metabolism, significant reduction of the culture media amount used, as well as researcher's temporary and labor efforts while the analyses realization, and the growth of analysis objectivity.


Keywords: biotesting, conductivity measurement, decontaminate activity, impedance analysis of microorganisms, microbiological growth curves, transglutaminaza, milk ripening

References
1. Labinskaya A.S. Mikrobiologiya s Tekhnikoi Mikrobiologicheskikh Iissledovanii [Microbiology with the Technique of Microbiological Studies]. Moscow, Meditsina Publ., 1972, 480 p.
2. Korotyaev A.I., Babichev A.S. Meditsinskaya Mikrobiologiya, Immunologiya i Virusologiya [Medical Microbiology, Immunology and Virology]. 4th ed. St. Petersburg, SpetsLit Publ., 2008, 767 p.
3. Gulya A.P., Prisakar V.I., Tsapkov V.I., Buracheva S.A., Spynu S.N., Bezhenar N.P. Synthesis and antimicrobial activity of sulfanilamide-containing copper (IInaphthalidenethiosemicarbazidates. Pharmaceutical Chemistry Journal, 2008, vol. 42, no. 6, pp. 326–328. doi: 10.1007/s11094-008-0118-6
4. Velikorodov A.V., Ionova V.A., Degtyarev O.V., Sukhenko L.T. Synthesis and antimicrobial and antifungal activity of carbamate-functionized spiro compounds. Pharmaceutical Chemistry Journal, 2013, vol. 46, no. 12, pp. 715–719. doi: 10.1007/s11094-013-0876-7
5. Yunnikova L.P., Akent'eva T.A., Aleksandrova G.A. Synthesis and antimicrobial activity of amines and imines with a cycloheptatriene fragment. Pharmaceutical Chemistry Journal, 2013, vol. 46, no. 12, pp. 723– 725. doi: 10.1007/s11094-013-0878-5
6. Vorob'eva A.I., Sultanova G.R., Bulgakov A.K., Zajnchkovskii V.I., Kolesov S.V. Synthesis and biological properties of copolymers based on N,N-diallyl-N,N-dimethylammonium chloride. Pharmaceutical Chemistry Journal, 2013, vol. 46, no. 11, pp. 653–655. doi: 10.1007/s11094-013-0863-z
7. Belakhov V.V., Shenin Yu.D. Synthesis and antifungal activity of N-trialkylsilyl derivatives of nystatin. Pharmaceutical Chemistry Journal, 2008, vol. 42, no. 6, pp. 322–325. doi: 10.1007/s11094-008-0117-7
8. Anikina L.V., Vikharev Yu.B., Rozhkova Yu.S., Shklyaev Yu.V. Synthesis and biological activity of 3- spiro[adamantane-2,3′-isoquinolines]. Pharmaceutical Chemistry Journal, 2013, vol. 46, no. 12, pp. 707– 710. doi: 10.1007/s11094-013-0874-9
9. Belen'kii M.L. Elementy Kolichestvennoi Otsenki Farmakologicheskogo Effekta [Elements of Quantitative Assessment of Pharmacological Effect]. Leningrad, Medgiz Publ., 1963, 146 p.
10. Raevsky O.A., Razdolskii A.N., Liplavskii Ya.V., Raevskaya O.E., Yarkov A.V. Acute toxicity evaluation upon intravenous injection into mice: interspecies correlations, lipophilicity parameters, and physicochemical descriptors. Pharmaceutical Chemistry Journal, 2012, vol. 46, no. 2, pp. 69–74. doi: 10.1007/s11094-012-
0736-x 11. Raevskii O.A., Razdol'skii A.N., Tonkopii V.D., Iofina I.V., Zagrebin A.O. Classificatory and quantitative models of the relationship between the structures of chemical compounds and their toxicity for Daphnia magna. Pharmaceutical Chemistry Journal, 2008, vol. 42, no. 6, pp. 329–334. doi: 10.1007/s11094-008-0119-5
12. Markosyan A.I., Akalyan Kh.S., Arsenyan F.G., Sukasyan R.S., Garibdzhanyan B.T. Synthesis and biological properties of 3-phenyl- and 3-phenethyl-5-methyl-5-ethyl-4-oxo-3,4,5,6-tetrahydrobenzo[h]quinazolines. Pharmaceutical Chemistry Journal, 2008, vol. 42, no. 6, pp. 313–318. doi: 10.1007/s11094-008-0115-9
13. Shakurova E.R., Parfenova T.I., Sufiyarova R.Sh., Khalilova A.Z., Akhmetova V.R., Bashkatov S.A. Synthesis and anti-inflammatory activity of acyl derivatives of taraxasterol. Pharmaceutical Chemistry Journal, 2008, vol. 42, no. 6, pp. 319–321. doi: 10.1007/s11094-008-0116-8
14. Kedik S.A., Sakaeva I.V., Kochkina Yu.V., Eremin D.V., Panov A.V., Suslov V.V. Synthesis and radioprotector activity of N-vinylpyrrolidone and 2-methyl-5-vinyltetrazole copolymers. Pharmaceutical Chemistry Journal, 2013, vol. 46, no. 12, pp. 726–729. doi: 10.1007/s11094-013-0879-4
15. Sibirtsev V.S. Study of applicability of the bifunctional system "ethidium bromide + Hoechst-33258" for DNA analysis. Biochemistry (Moscow), 2005, vol. 70, no. 4, pp. 449–457. doi: 10.1007/s10541-005-0136-x
16. Sibirtsev V.S. Fluorescent DNA probes: study of mechanisms of changes in spectral properties and features of practical application. Biochemistry (Moscow), 2007, vol. 72, no. 8, pp. 887–900. doi: 10.1134/S0006297907080111
17. Sibirtsev V.S., Garabadzhiu A.V. Fluorestsentnye DNK-Zondy: Vvedenie v Teoriyu i Praktiku Ispol'zovaniya [Fluorescent DNA Probes: An Introduction to the Theory and Practice of Using]. St. Petersburg, NOU Ekspress Publ., 2006, 188 p.
18. Sibirtsev V.S., Garabadzhiu A.V. Novye metody biotestirovaniya i analiza DNK s pomoshch'yu fluoroforov [New methods of bioassay and DNA analysis using fluorophores]. Biotekhnosfera, 2011, vol. 13, no. 3, pp. 9–14.
19. Bessonov L.A. Teoreticheskie Osnovy Elektrotekhniki [Theory of Electrical Engineering]. Moscow, Vysshaya Shkola Publ., 1996, 492 p.
20. Shleikin A.G., Krasnikova L.V., Danilov N.P. Substrate specificity of transglutaminase. Influence of transglutaminase on milk whey proteins cross-linking. In: Food Technology Operations / Eds W. Kopec, M. Korzeniowska. Wroclaw, New Vistas, 2009, 317 p.
Copyright 2001-2017 ©
Scientific and Technical Journal
of Information Technologies, Mechanics and Optics.
All rights reserved.

Яндекс.Метрика