doi: 10.17586/2226-1494-2017-17-2-263-268


L. V. Plotnikova, A. M. Polyanichko, M. O. Kobeleva, M. V. Uspenskaya, A. D. Garifullin, S. V. Voloshin

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For citation: Plotnikova L.V., Polyanichko A.M., Kobeleva M.O., Uspenskaya M.V., Garifullin A.D., Voloshin S.V. Refractometric analysis of proteinemia level and serum electrolyte imbalance of patients with multiple myeloma. Scientific and Technical Journal of Information Technologies, Mechanics and Optics, 2017, vol. 17, no. 2, pp. 263–268 (in Russian). doi: 10.17586/2226-1494-2017-17-2-263-268.


Subject of Research. Application of refractometric analysis in multiple myeloma diagnostics is proposed for the first time. Multiple myeloma (MM) is a disease of the hematopoietic system characterized by active monoclonal protein synthesis being detected in blood serum and/or in urine. Method. The essence of method consists in measuring of the sample refractive index. The value of refractive index depends on physicochemical characteristics of the optical mediums. Differences between refractive index of patients with multiple myeloma and donor blood serum connected with typical protein and electrolyte imbalance were researched. Main Results. Refractometric analysis data have shown strong correlations between protein concentrations in serum of patients with multiple myeloma and serumrefractive index. Refractive index of serum of patients with multiple myeloma firstly depends on protein concentration rather than on an electrolyte imbalance. The main tendency for patients with multiple myeloma is a higher level of both protein and electrolytes concentration in serum, in contrast to healthy people. Practical Relevance. According to the obtained data, refractometric analysis is considered as one of the suitable methodsfor express-diagnostics of diseases, in particular, multiple myeloma.

Keywords: refractometric analysis, multiple myeloma, refractive index, M-protein, electrolytes

 1.     Clinical Oncohematology. Ed. M.A. Volkova. Moscow, Meditsina Publ., 2001, 576 p. (In Russian)
2.     Saraeva N.O. Leukemia. Irkutsk, 2009, 122 p. (In Russian)
3.     Serov V.V., Pal'tsev M.A. Pathological Anatomy. Lecture Course. Moscow, Meditsina Publ., 1998, 640 p. (In Russian)
4.     Gertz M.A., Rajkumar S.V. Multiple Myeloma. Diagnosis and Treatment. NY, Springer, 2014, 311 p. doi: 10.1007/978-1-4614-8520-9
5.     Munshi N., Anderson K. Advances in Biology and Therapy of Multiple Myeloma. NY, Springer, 2013, vol. 1, 319 p.
6.     Devenney B., Erickson Ch. Multiple myeloma: an overview. Clinical Journal of Oncology Nursing, 2004, vol. 8, no. 4, pp. 401–405.
7.     Mohty M., Harousseau J.-L. Handbook of Multiple Myeloma. Adis, 2015, 90 p.
8.     Pop V.P., Kozlov G.K., Pravosudov V.V. et. al. Multiple Myeloma and Related Diseases. 3rd ed. Moscow, GEOTAR-Media, 2016, 224 p. (In Russian)
9.     Svistunov A., Ruta A., Shevchenko O. Peculiarities of bone metabolism in patients with multiple myeloma. Saratov Journal of Medical Scientific Research, 2010, no. 6, pp. 48–57. (In Russian)
10.  Abraham R., Clark R., Bryant S., Lymp J., Larson T., Kyle R., Katzmann J. Correlation of serum immunoglobulin free light chain quantification with urinary Bence-Jones protein in light chain myeloma. Clinical Chemistry, 2002, vol. 48, no. 4, pp. 655–657.
11.  Kalyuzhnyi V.P. Normal and pathological electrolytes and methods of their investigation. Terra Medica, 2003, no. 1, pp. 30–32. (In Russian)
12.  Plotnikova L., Polyanichko A., Nosenko T., Uspenskaya M., Garifullin A., Voloshin S. Characterization of the infrared spectra of serum from patients with multiple myeloma. AIP Conference Proceedings, 2016,vol. 1760, art. 020052. doi:10.1063/1.4960271 
13.  Plotnikova L.V., Polyanichko A.M, Uspenskaya M.V., Garifullin A.D., Voloshin S.V. Features of infrared spectra of blood serum for multiple myeloma patients. Rossiiskii Bioterapevticheskii Zhurnal, 2016, vol. 15, no. 1, pp. 87–88.(In Russian)
14.  Polyanichko A.M., Romanov N.M., Starkova T.Y., Kostyleva E.I., Chikhirzhina E.V. Analysis of the secondary structure of linker histone H1 based on IR absorption spectra. CellandTissueBiology, 2014,vol. 8, no. 4, pp. 352–358. doi: 10.1134/s1990519x14040087 
15.  Stifatov B.M., Rublinetskaya Yu.V. Refractometry. Samara: SamSTU Publ., 2013, 15 p. (In Russian)
16.  Annesley T., Burritt M., Kyle R. Artifactual hypercalcemia in multiple myeloma. Mayo Clinic Proceedings, 1982, vol. 57, no. 9, pp. 572–586
17.  Gertz M., Greipp P. Hematologic Malignancies: Multiple Myeloma and Related Plasma Cell Disorders. NY, Springer, 2004, 272 p.
18.  Wiernik P., Goldman J., Dutcher J., Kyle R. Neoplastic Diseases of the Blood. NY,Springer, 2013, 1431 p. doi: 10.1007/978-1-4614-3764-2
19.  Cheknev S.B., Babaeva E.E., Golub A.E., Denisova E.A., Vorob'eva U.A. Effects of copper and zinc on binding to human serum gamma globulin. Meditsinskaya Immunologiya, 2006, no. 8, pp. 615–622.(In Russian)
20.  Ryzhko V.V., Klodzinskii A.A., Varlamova E.Yu., Sataeva M.S., Kaplanskaya I.B., Nakastoev I.M., Paraskevova O.V. «Nonsecretory» multiple myeloma (a review of literature and own data). Clinical Oncohematology, 2010, no. 3, pp. 270–277.(In Russian)
21.  Bessmeltsev S.S. Multiple myeloma (pathogenesis, clinical features, diagnosis, differential diagnosis). Part I. Clinical Oncohematology, 2013, no. 6, pp. 237–257. (In Russian)
22.  Kravchenko D.V., Khoduleva S.A., Novik D.K. Multiple Myeloma. Gomel', RNPTs RMiECh, 2016, 83 p. (In Russian)

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