FOURIER SPECTROSCOPY IN BLOOD PLASMA STUDY WITH TYPE TWO DIABETES

Nechiporenko A.P., Nechiporenko U.Yu., Sitnikova V.E. Fourier spectroscopy in blood plasma study with type two diabetes. Scientific and Technical Journal of Information Technologies, Mechanics and Optics, 2021, vol. 21, no. 1, pp. 52–64 (in Russian). doi: 10.17586/2226-1494-2021-21-1-52-64

Subject of Research. The paper presents the study of possibilities of a spectral technique for evaluating changes in the optical properties of carbohydrates and plasma proteins in humans with the initial stage of type two diabetes. The optical characteristics are compared with those obtained by the conventional method of sugar curves using sucrose, honey and milk protein as a provoking load at various stages of treatment with antidiabetic drugs. Method. The study was carried out by infrared spectroscopy of disturbed total internal reflection in the range of 4000–500 cm^–1. Testing for glucose tolerance at all stages of treatment was performed by the biochemical glucose oxidase method. Main Results. The use of provocateur products of various nature and an extended glucose tolerance test makes it possible to identify the spectrum bands associated with the presence of glucose (1104 cm^–1) and fructose (1115 cm^–1), differentiating on the left branch of the complex carbohydrate band (1075 cm^–1) of the infrared spectrum of native plasma. It is shown that the change in the intensity of the Amide-I and Amide-II bands of fractionated plasma proteins is associated with the main glucose transporter — globulin fraction proteins. Practical Relevance. The revealed features of changes in blood plasma spectra in the course of the conducted studies give reason to believe that the non-destructive method of Fourier spectroscopy does not require a large volume of the studied material and its preliminary sample preparation. The method is promising as an express tool and can be used to obtain additional information when studying the influence of various provoking factors on the nature of changes in the optical characteristics of protein-lipid-carbohydrate complexes and globular proteins of blood plasma, as well as for preliminary diagnosis and the treatment supervision of type two diabetes mellitus.

1. Stuart B.H. Infrared Spectroscopy: Fundamentals and Applications. N.-Y., Wiley, 2004, 242 p.

2. Larkin P.J. Infrared and Raman Spectroscopy: Principles and Spectral Interpretation. Elsevier, 2011, 230 p.

3. Harrik N. Internal Reflection Spectroscopy. Interscience Publishers, 1967, 327 p.

4. Bell R.J. Introductory Fourier Transform Spectroscopy. Academic Press, 1972, 382 p.

5. Tarasevich B.N. Fundamentals of IR spectroscopy with Fourier transform. Sample preparation in IR spectroscopy. Moscow, MSU, 2010, 55 p. (in Russian)

6. Landsberg G.S. Optics. Tutorial. 6th ed. Moscow, Fizmatlit Publ., 2010, 848 p. (in Russian)

7. Kuzmin V.S., Apanasevich E.E., Bokataia E.L., Fedorenchik E.V. General Physics. Optics. Physics. Optics. Minsk, ISEI BSU, 2010, 72 p. (in Russian)

8. Pretsch E., Bühlmann Ph., Affolter Ch. Determination of the Structure of Organic Compounds. Tables of Spectral Data. Berlin, Springer, 2000, 421 p. doi: 10.1007/978-3-662-04201-4

9. Sidorenko V.M. Application of Optical Spectral Research Methods in Medicine, Biology and Ecology. St. Petersburg, ETU “LETI”, 2001, 25 p. (in Russian)

10. Trineeva O.V., Rudaya M.A., Gudkova A.A., Slivkin A.I. Application of IR-spectroscopy in the analysis of vegetable plant raw material. Proceedings of Voronezh State University. Series: Chemistry. Biology. Pharmacy, 2018, no. 4, pp. 187–194. (in Russian)

11. Perisic N., Afseth N.K., Ofstad R., Narum B., Kohler A. Characterizing salt substitution in beef meat processing by vibrational spectroscopy and sensory analysis. Meat Science, 2013, vol. 95, no. 3, pp. 576–585.

doi: 10.1016/j.meatsci.2013.05.043

12. Barannikova I.N. The use of FTIR spectroscopy in forensic practice and its potential future applications. Theory and Practice of Forensic Science, 2017, vol. 12, no. 1, pp. 85–91. (in Russian)

13. Suntsova A.Yu., Guliev R.R., Popov D.A., Vostrikova T.Yu., Dubodelov D.V., Shchegolikhin A.N., Laypanov B.K., Priputnevich T.V., Shevelev A.B., Kurochkin I.N. Identification of microorganisms by Fourier-transform infrared spectroscopy. Bulletin of Russian State Medical University, 2018, vol. 7, no. 4, pp. 50–57. doi: 10.24075/brsmu.2018.046

14. Srividya P., Renuga Devi T.S., Gunasekaran S. FTIR spectral study on diabetic blood samples-monotherapy and combination therapy. OJP, 2012, vol. 4, no. 1, pp. 17–26.

15. Petrich W., Staib A., Otto M., Somorjai R.L. Correlation between the state of health of blood donors and the corresponding mid-infrared spectra of the serum. Vibrational Spectroscopy, 2002, vol. 28, no. 1, pp. 117–129.

doi: 10.1016/S0924-2031(01)00151-5

16. Lumi E., Pine S., Pengo P., Puca E., Agron Y. The incidence rate of diabetes mellitus and pre-diabetes in Korca-Albania, in 2015. Medico Research Chronicles, 2016, vol. 3, no. 2, pp. 176–182.

17. Mediani A., Abas F., Maulidiani M., Abu Bakar Sajak A., Khatib A., Tan C.P., Ismail I.S., Shaari K., Ismail A., Lajis N.H. Metabolomic analysis and biochemical changes in the urine and serum of streptozotocin-induced normal- and obese-diabetic rats. Journal of Physiology and Biochemistry, 2018, vol. 74, no. 3, pp. 403–416. doi: 10.1007/s13105-018-0631-3

 18. Nosenko T.N., Sitnikova V.E., Olekhnovich R.O., Uspenskaya M.V. Detection of diabetes mellitus type II by infrared spectroscopy and multivariate analysis. Scientific and Technical Journal of Information Technologies, Mechanics and Optics, 2019, vol. 19, no. 2, pp. 202– 208. (in Russian). doi: 10.17586/2226-1494-2019-19-2-202-208

19. Misnikova I.V., Dreval A.V., Gubkina V.A., Kovaleva Yu.A. Algorithm for Diagnosis of Type 2 Diabetes and Control of Carbohydrate Metabolism. Moscow, GBUZ MONIKI, 2015, 28 p. (in Russian)

20. Bugrov A.V., Dolgov V.V., Kazakov S.P. et al. Clinical Laboratory Diagnostics. V. 1. Moscow, LabDiag Publ., 2017, 464 p. (in Russian)

21. Diabetes Mellitus. Diagnostics. Treatment. Prevention. Ed. by I.I. Dedov, M.V Shestakova. Moscow, Medical Information Agency, 2011, 808 p. (in Russian)

22. Shleikin A.G., Skvortcova N.N., Blandov A.N. Biochemistry. Laboratory   Practice.   Part   2.   Proteins.   Enzymes.   Vitamins. St. Petersburg, ITMO University, 2015, 106 p. (in Russian)

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