doi: 10.17586/2226-1494-2020-20-6-791-801


EFFECT OF PLANT EXTRACTS ON ACTIVITY OF STAPHYLOCOCCUS AUREUS BY ELECTROCHEMICAL BIOTESTING

V. S. Sibirtsev, U. Y. Nechiporenko, V. L. Kabanov, M. Y. Kukin, A. Y. Maslova, M. A. Radin


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Sibirtsev V.S., Nechiporenko U.Yu., Kabanov V.L., Kukin M.Yu., Maslova A.Yu., Radin M.A. Effect of plant extracts on activity of Staphylococcus aureus by electrochemical biotesting. Scientific and Technical Journal of Information Technologies, Mechanics and Optics, 2020, vol. 20, no. 6, pp. 791-801 (in Russian). doi: 10.17586/2226-1494-2020-20-6-791-801


Abstract
Subject of Research. The paper presents a developed method for rapid instrumental assessment of microbial contamination, as well as pro- and antibiotic properties of pharmacological, food and other products. Method. The developed technique consisted in periodic instrumental registration of changes in pH, redox potential, and electrolytic conductivity of a liquid nutrient medium incubated in the presence and absence of viable test microorganisms and test samples. A comparative analysis of the pro- and antibiotic activity of Staphylococcus aureus was carried out at various concentrations of whole subcritical extracts obtained from ten different types of plant raw materials using liquefied carbon dioxide as an extractant. Main Results. The studies carried out have confirmed that the presented method makes it possible to assess the initial microbial contamination more speedily, objectively and informatively in comparison with the standard one as well as the effect of various samples of pharmaceutical, food and other products on the dynamics of the microorganisms’ vital activity. Among the studied samples, the most active prolonged antibiotic properties have been exhibited by extracts from the fruits of Sambucus nigra and Rosa cinnamomea, as well as green leaves of Camellia sinensis at their concentrations in the test medium from 3 vol.% and higher. The most active prolonged probiotic properties have been found in extracts from the Hypericum perforatum herb and green leaves of Camellia sinensis at their concentrations in the test medium equal to 0.2 vol.%. In this case, the biological activity of the tested samples with respect to test microorganisms in the most cases have monotonically decreased with an increase in the interaction time of the mentioned microorganisms and samples. Practical Relevance. The results of this study can find application in the composition development and assessment of the properties of new pharmaceutical, food and other products, including various plant extracts. In addition, the proposed method of instrumental microbiological testing can be applicable for control of microbial contamination, as well as pro- and antibiotic properties of various samples of products already accepted for the usage, and individual ingredients and additives to them.

Keywords: microbiological biotesting, antibiotic properties, plant extracts, microbial contamination

References
1. Sutherland J., Miles M., Hedderley D., Li J., Devoy S., Sutton K., Lauren D. In vitro effects of food extracts on selected probiotic and pathogenic bacteria. International Journal of Food Sciences and Nutrition, 2009, vol. 60, no. 8, pp. 717–727. doi: 10.3109/09637480802165650
2. Das S., Anjeza C., Mandal S. Synergistic or additive antimicrobial activities of Indian spice and herbal extracts against pathogenic, probiotic and food-spoiler micro-organisms. International Food Research Journal, 2012, vol. 19, no. 3, pp. 1185–1191.
3. Al-Zubairi A.S., Al-Mamary M.A., Al-Ghasani E. The antibacterial, antifungal, and antioxidant activities of essential oil from different aromatic plants. Global Advanced Research Journal of Medicine and Medical Sciences, 2017, vol. 6, no. 9, pp. 224–233.
4. Rodino S., Butu M. Herbal extracts-new trends in functional and medicinal beverages. Functional and Medicinal Beverages. Volume 11: The Science of Beverages, 2019, pp. 73–108. doi: 10.1016/B978-0-12-816397-9.00003-0
5. Burt S. Essential oils: their antibacterial properties and potential applications in foods — a review. International Journal of Food Microbiology, 2004, vol. 94, no. 3, pp. 223–253. doi: 10.1016/j.ijfoodmicro.2004.03.022
6. Bakkali F., Averbeck S., Averbeck D., Idaomar M. Biological effects of essential oils — a review. Food and Chemical Toxicology, 2008, vol. 46, no. 2, pp. 446–475. doi: 10.1016/j.fct.2007.09.106
7. Tripathi A.K., Bhoyar P.K., Baheti J.R., Biyani D.M., Khalique M., Kothmire M.S., Amgaonkar Y.M., Bhanarkar A.B. Herbal antidiabetics: a review. International Journal of Research in Pharmaceutical Sciences, 2011, vol. 2, no. 1, pp. 30–37.
8. Fatima A., Alok S., Agarwal P., Singh P.P., Verma A. Benefits of herbal extracts in cosmetics: a review. International Journal of Pharmaceutical Sciences and Research, 2013, vol. 4, no. 10, pp. 3746–3760. doi: 10.13040/ijpsr.0975-8232.4(10).3746-60
9. Alok S., Jain S.K., Verma A., Kumar M., Mahor A., Sabharwal M. Herbal antioxidant in clinical practice: a review. Asian Pacific Journal of Tropical Biomedicine, 2014, vol. 4, no. 1, pp. 78–84. doi: 10.1016/S2221-1691(14)60213-6
10. Radice M., Manfredini S., Ziosi P., Dissette V., Buso P., Fallacara A., Vertuani S. Herbal extracts, lichens and biomolecules as natural photo-protection alternatives to synthetic UV filters. A systematicreview. Fitoterapia, 2016, vol. 114, pp. 144–162. doi: 10.1016/j.fitote.2016.09.003
11. Merghni A., Marzouki H., Hentati H., Aouni M., Mastouri M. Antibacterial and antibiofilm activities of Laurus nobilis L. essential oil against Staphylococcus aureus strains associated with oral infections. Current Research in Translational Medicine, 2016, vol. 64, no. 1, pp. 29–34. doi: 10.1016/j.patbio.2015.10.003
12. Fani M., Kohanteb J. In vitro antimicrobial activity of Thymus vulgaris essential oil against major oral pathogens. Journal of Evidence-Based Complementary and Alternative Medicine, 2017, vol. 22, no. 4, pp. 660–666. doi: 10.1177/2156587217700772
13. Kokina M.S., Frioui M., Shamtsyan M., Sibirtsev V.S., Krasnikova L.V., Konusova V.G., Simbirtsev A.S. Influence of pleurotus ostreatus β-glucans on the growth and activity of certain lactic acid bacteria. Scientific Study and Research: Chemistry and Chemical Engineering, Biotechnology, Food Industry, 2018, vol. 19, no. 4, pp. 465–471.
14. Atarés L., Chiralt A. Essential oils as additives in biodegradable films and coatings for active food packaging. Trends in Food Science & Technology, 2016, vol. 48, pp. 51–62. doi: 10.1016/j.tifs.2015.12.001
15. Ribeiro-Santos R., Andrade M., Melo N.R., Sanches-Silva A. Use of essential oils in active food packaging: Recent advances and future trends. Trends in Food Science & Technology, 2017, vol. 61, pp. 132– 140. doi: 10.1016/j.tifs.2016.11.021
16. Ju J., Xie Y., Guo Y., Cheng Y., Qian H., Yao W. Application of edible coating with essential oil in food preservation. Critical Reviews in Food Science and Nutrition, 2019, vol. 59, no. 15, pp. 2467–2480. doi: 10.1080/10408398.2018.1456402
17. Yuan G., Chen X., Li D. Chitosan films and coatings containing essential oils: The antioxidant and antimicrobial activity, and application in food systems. Food Research International, 2016, vol. 89, pp. 117–128. doi: 10.1016/j.foodres.2016.10.004
18. Donsì F., Ferrari G. Essential oil nanoemulsions as antimicrobial agents in food. Journal of Biotechnology, 2016, vol. 233, pp. 106– 120. doi: 10.1016/j.jbiotec.2016.07.005
19. Pavela R., Benelli G. Essential oils as ecofriendly biopesticides? Challenges and constraints. Trends in Plant Science, 2016, vol. 21, no. 12, pp. 1000–1007. doi: 10.1016/j.tplants.2016.10.005
20. Rout P.K., Naik S.N., Rao Y.R. Subcritical CO2 extraction of floral fragrance from Quisqualis indica. Journal of Supercritical Fluids, 2008, vol. 45, no. 2, pp. 200–205. doi: 10.1016/j.supflu.2008.02.011
21. Sahena F., Zaidul I.S.M., Jinap S., Karim A.A., Abbas K.A., Norulaini N.A.N., Omar A.K.M. Application of supercritical CO2 in lipid extraction — A review. Journal of Food Engineering, 2009, vol. 95, no. 2, pp. 240–253. doi: 10.1016/j.jfoodeng.2009.06.026
22. Ibadullaeva G.S., Pichkhadze G.M., Ustenova G.O., Dil’barkhanov R., Tikhonova S.A., Grud’ko V.A., Bevz N.Yu., Yudina Yu.V. Chemical composition of the CO2-extract of Acorus calamus obtained under subcritical conditions. Pharmaceutical Chemistry Journal, 2015, vol. 49, no. 6, pp. 388–392. doi: 10.1007/s11094-015-1290-0
23. Valle D.L.,Jr., Cabrera E.C., Puzon J.J.M., Rivera W.L. Antimicrobial activities of methanol, ethanol and supercritical CO2 extracts of Philippine Piper betle L. on clinical isolates of Gram positive and Gram negative bacteria with transferable multiple drug resistance. PLoS ONE, 2016, vol. 11, no. 1, pp. e0146349. doi: 10.1371/journal. pone.0146349
24. Lazarotto M., Valério A., Boligon A., Tres M.V., Scapinello J., Dal Magro J., Oliveira J.V. Chemical composition and antibacterial activity of bergamot peel oil from supercritical CO2 and compressed propane extraction. Open Food Science Journal, 2018, vol. 10, no. 1, pp. 16–23. doi: 10.2174/1874256401810010016
25. Vieitez I., Maceiras L., Jachmanián I., Alborés S. Antioxidant and antibacterial activity of different extracts from herbs obtained by maceration or supercritical technology. Journal of Supercritical Fluids, 2018, vol. 133, pp. 58–64. doi: 10.1016/j.supflu.2017.09.025
26. Coelho J., Veiga J., Karmali A., Nicolai M., Reis C.P., Nobre B., Palavra A. Supercritical CO2 extracts and volatile oil of basil (Ocimum basilicum L.) comparison with conventional methods. Separations, 2018, vol. 5, no. 2, pp. 21. doi: 10.3390/separations5020021
27. Sibirtsev V.S., Naumov I.A., Kuprina E.E., Olekhnovich R.O. Use of impedance biotesting to assess the actions of pharmaceutical compounds on the growth of microorganisms. Pharmaceutical Chemistry Journal, 2016, vol. 50, no. 7, pp. 481–485. doi: 10.1007/s11094-016-1473-3
28. Sibirtsev V.S., Ignatjeva A.F., Shichkova K.A., Tran Thanh Tuan, Stroev S.A., Radin M.A. Study of influence of the highfrequency electric fields on microbial vital activity at various temperatures. Scientific and Technical Journal of Information Technologies, Mechanics and Optics, 2017, vol. 17, no. 2, pp. 279–286. (in Russian). doi: 10.17586/2226-1494-2017-17-2-279-286
29. Sibirtsev V.S., Olekhnovich R.О., Samuylova E.О. Assessment of integral toxicity of water resources by instrumental methods of analysis. Proc. 17th International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management (SGEM), 2017, vol. 17, no. 61, pp. 507–514. doi: 10.5593/sgem2017/61/S24.066
30. Sibirtsev V.S. Biological test methods based on fluorometric genome analysis. Journal of Optical Technology, 2017, vol. 84, no. 11, pp. 787–791. doi: 10.1364/JOT.84.000787
31. Sibirtsev V.S., Uspenskaya M.V., Garabadgiu A.V., Shvets V.I. An integrated method of instrumental microbiotesting of environmental safety of various products, wastes, and territories. Doklady Biological Sciences, 2019, vol. 485, no. 1, pp. 5 9 – 6 1 . doi: 10.1134/S001249661902011X
32. Sibirtsev V.S., Stroev S.A. New optical-electrochemical microbiotesting system for valuation of oil products toxicosafety. Scientific and Technical Journal of Information Technologies, Mechanics and Optics, 2019, vol. 19, no. 1, pp. 74–81. (in Russian). doi: 10.17586/2226-1494-2019-19-1-74-81
33. Sibirtsev V.S., Maslova A.Yu. Complex research of E.coli vital activity dynamics in presence of transition metal ions. Scientific and Technical Journal of Information Technologies, Mechanics and Optics, 2019, vol. 19, no. 2, pp. 236–241 (in Russian). doi: 10.17586/2226-1494-2019-19-2-236-241
34. Sibirtsev V.S., Garabadgiu A.V., Shvets V.I. New method of integrated photofluorescence microbiotesting. Doklady Biological Sciences, 2019, vol. 489, no. 6, pp. 196–199. doi: 10.1134/S0012496619060103
35. Korn G.A., Korn T.M. Mathematical Handbook for Scientists and Engineers. Definitions, Theorems and Formulas for Reference and Review. McGraw Hill, 1968, 1130 p.
36. Johnson K., Jeffi V. Numerical Methods in Chemistry. New York, Cambridge University Press, 1983.
37. Sibirtsev V.S. Analysis of benzo[a]pyrene deactivation mechanisms in rats. Biochemistry (Moscow), 2006, vol. 71, no. 1, pp. 90–98. doi: 10.1134/S0006297906010147
38. Zhuravlev O.E., Voronchikhina L.I. Synthesis and antimicrobial activity of N-decylpyridinium salts with inorganic anions. Pharmaceutical Chemistry Journal, 2018, vol. 52, no. 4, pp. 312–315. doi: 10.1007/s11094-018-1813-6
39. Luzhnova S.A., Tyrkov A.G., Gabitova N.M., Yurtaeva E.A. Synthesis and antimicrobial activity of 5-(arylmethylidene)-2,4,6-pyrimidine2,4,6(1H,3H,5H)-triones. Pharmaceutical Chemistry Journal, 2018, vol. 52, no. 6, pp. 506–509. doi: 10.1007/s11094-018-1849-7


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