doi: 10.17586/2226-1494-2016-16-3-573-576


V. S. Sibirtsev, A. Y. Kulakov, S. A. Stroev

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For citation: Sibirtsev V.S., Kulakov A.Yu., Stroev S.A. Conductometry biotesting as applied to valuation of the pro- and antibacterial properties of catolites and anolites. Scientific and Technical Journal of Information Technologies, Mechanics and Optics, 2016, vol. 16, no. 3, pp. 573–576. doi: 10.17586/2226-1494-2016-16-3-573-576


The paper deals with technique of the electrical conductivity biotesting as applied to the analysis of pro- and antibacterial activity of catolit and anolit solutions. The tested preparations were received by 25 minute electrolysis processing of 1% NaCl water solution (in cathode space) and 1% Na2SO4 water solution (in anode space) at 7 A amperage and 28 V voltage. The submitted technique is based on the analysis of change dynamics of impedance electrical conductivity of researched samples, caused by metabolic processes, realized by tested microorganisms in analyzed samples. Application of this technique enabled to show that makeup anolit solution, beginning from concentration equal to 1 vol. %, has a bacteriostatic effect on liquid culture medium with Escherichia coli in the quantity of 104 live cells on 1 ml. At anolit solution concentration more than 5 vol.% its action on the same microorganismes becomes bactericidal. At the same time, makeup catolit solution activates microorganismes vital activity as early as from concentration equal to 5 vol.%. Thus, it is shown, that electrical conductivity biotesting is a sensitive laboratory tool, granting accessible, convenient and informative way to a researcher for valuation of properties of various pro- and anti-infective preparations, as well as the other physico-chemical systems (including rather complex concerning structure and dynamics of its change), capable to act on microorganismes vital activity.

Keywords: biotesting, conductivity measurement, antiseptic properties, catolites and anolites, pro- and antibacterial activity


1. Zvarich V.I., Stasevich M.V., Stan’ko O.V., Komarovskaya-Porokhnyavets E.Z., Poroikov V.V., Rudik A.V., Lagunin A.A., Vovk M.V., Novikov V.P. Computerized prediction, synthesis, and antimicrobial activity of new amino-acid derivatives of 2-chloro-n-(9,10-dioxo-9,10-dihydro-anthracen-1-yl) acetamide. Pharmaceutical Chemistry Journal, 2015, vol. 48, no. 9, pp. 582–586. doi: 10.1007/s11094-014-1154-z
2. Surikova O.V., Mikhailovskii A.G., Odegova T.F. Synthesis and antimicrobial and antifungal activities of 3-substituted 1-cyanomethyl-3,4-dihydroisoquinolinium chlorides. Pharmaceutical Chemistry Journal, 2015, vol. 48, no. 11, pp. 711–713. doi: 10.1007/s11094-015-1178-z
3. Madesclaire M., Coudert P., Lyamin A.V., Sharipova S.Kh., Zaitseva Yu.V., Zaitsev V.P. Synthesis and antimicrobial activity of new ureas from (1s,2s)-2-amino-1-(4-nitrophenyl)-1,3-propanediol. Pharmaceutical Chemistry Journal, 2015, vol. 49, no. 1, pp. 10–12. doi: 10.1007/s11094-015-1213-0
4. Popov L.D., Levchenkov S.I., Zubenko A.A., Shcherbakov I.N., Fetisov L.N., Bodryakov A.N., Maevskii O.V., Kogan V.A. Synthesis, protistocidal and antibacterial activities of 2′-imidazolinylhydrazones of mono- and dicarboxylic acids. Pharmaceutical Chemistry Journal, 2015, vol. 49, no. 1, pp. 21–23. doi: 10.1007/s11094-015-1215-y
5. Divaeva L.N., Klimenko A.I., Morkovnik A.S., Fetisov L.N., Kuz’menko T.A., Zubenko A.A., Bodryakova M.A., Bodryakov A.N. Synthesis and antimicrobial and protistocidal activity of 1-(2-aryloxyethyl- and 2-halobenzyl)-3-(2-hydroxyethyl)-2-imino-1,3-dihydrobenzimidazolines. Pharmaceutical Chemistry Journal, 2015, vol. 49, no. 2, pp. 91–95. doi: 10.1007/s11094-015-1228-6
6. Odaryuk V.V., Burakov N.I., Kanibolotskaya L.V., Kanibolotskii A.L., Odaryuk I.D., Lebedeva N.Yu., Poddubnaya E.N., Shendrik A.N. Synthesis and antiradical and antibacterial activity of 4-(3′,4′-dihydroxyphenyl)thiazole derivatives. Pharmaceutical Chemistry Journal, 2015, vol. 49, no. 2, pp. 96–98. doi: 10.1007/s11094-015-1229-5
7. Grigor’eva M.N., Stel’makh S.A., Astakhova S.A., Tsenter I.M., Bazaron L.U., Batoev V.B., Mognonov D.M. Synthesis of polyalkylguanidine hydrochloride copolymers and their antibacterial activity against conditionally pathogenic microorganisms Bacillus Cereus and Escherichia Coli. Pharmaceutical Chemistry Journal, 2015, vol. 49, no. 2, pp. 99–103. doi: 10.1007/s11094-015-1230-z
8. Borisov L.B. Meditsinskaya Mikrobiologiya, Virusologiya i Immunologiya [Medical Microbiology, Virology and Immunology]. Moscow, MIA Publ., 2005, 743 p.
9. Produkty Pishchevye, Konservy. Metody Mikrobiologicheskogo Analiza: Sbornik GOSTov [Food Products, Canned Food. Methods of Microbiological Analysis: Collection of State Standards]. Moscow, Standartinform Publ., 2010, 463 p.
10. Mironov A.N., Bunatyan N.D. et. al. Rukovodstvo po Provedeniyu Doklinicheskikh Issledovanii Lekarstvennykh Sredstv [Guidelines for Preclinical Studies of Drugs]. Moscow, Grif i K Publ., 2012, 944 p.
11. Sibirtsev V.S., Krasnikova L.V., Schleikin A.G., Stroev S.A., Naumov I.A., Olekhnovich R.O., Tereschenko V.F., Shabanova E.M., Mussa Al-Khatib. New biotesting method with the application of modern impedance technologies. Scientific and Technical Journal of Information Technologies, Mechanics and Optics, 2015, vol.15, no. 2, pp. 275–284. doi: 10.17586/2226-1494-2015-15-2-275-284

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